Vol. 7, Issue 2, Part B (2025)

Background: Camellia sinensis (L.) Kuntze (green tea) is renowned for its health benefits, primarily attributed to its rich polyphenol content. While its leaves are extensively studied, a comprehensive phytochemical and pharmacological evaluation of its bioactivity remains relevant for validating traditional claims and exploring its nutraceutical potential.
Objective: This study aimed to conduct a systematic evaluation of the bioactive properties of a methanolic extract of Camellia sinensis leaves through phytochemical profiling and assessment of its antioxidant, antidiabetic, and antitumor activities.

Methods: The extract was prepared using methanol and subjected to qualitative phytochemical screening. Its antioxidant capacity was evaluated via a hydrogen peroxide (H₂O₂) scavenging assay, antidiabetic potential was assessed using a yeast cell glucose uptake model, and antitumor activity was investigated via the potato disc bioassay against Agrobacterium tumefaciens.

Results: Phytochemical analysis confirmed the presence of terpenoids, flavonoids, alkaloids, carbohydrates, phenolic compounds, and tannins. The extract demonstrated significant, dose-dependent antioxidant activity with an IC₅₀ value of 127.41 µg/mL. It also enhanced glucose uptake in yeast cells comparably to metformin and exhibited potent dose-dependent antitumor activity, achieving 100% inhibition of tumor formation at the highest concentration (750 µL).

Conclusion: The findings scientifically validate the traditional use of green tea, highlighting its multifaceted pharmacological potential. The synergistic action of its diverse phytochemicals underpins its strong antioxidant, antidiabetic, and antitumor properties, positioning it as a promising candidate for developing natural therapeutic adjuvants and nutraceuticals.