Vol. 7, Issue 1, Part C (2025)

Background and Aim: Parkinson's illness (PD) could be a inveterate, dynamic neurodegenerative clutter characterized primarily by the degeneration of dopaminergic neurons within the substantia nigra pars compacta of the midbrain. This neuronal misfortune comes about in a noteworthy diminishment in striatal dopamine levels, showing in both engine symptoms-such as resting tremor, bradykinesia, unbending nature, and postural instability-and non-motor symptoms counting misery, cognitive decrease, olfactory brokenness, and rest unsettling influences. The precise etiology of PD remains vague, but it is accepted to emerge from a combination of hereditary defenselessness and natural exposures.
A key obsessive trademark of PD is the nearness of Lewy bodies composed of misfolded alpha-synuclein protein totals, contributing to neuronal brokenness and cell passing. Besides, oxidative stretch, mitochondrial brokenness, neuroinflammation, and apoptotic pathways are intensely embroiled in PD pathogenesis. Current helpful strategies, including the utilize of levodopa and dopamine agonists, center essentially on symptomatic alleviation and don't offer a remedy or end infection movement. Additionally, long-term utilize of these drugs often results in unfavorable impacts such as engine vacillations and dyskinesia.
Given the restrictions of existing medicines, there's a developing intrigued in investigating elective neuroprotective specialists, especially from common sources. Restorative plants wealthy in phytochemicals like flavonoids, saponins, phenolics, and terpenoids offer promising neuroprotective potential due to their antioxidant and anti-inflammatory properties.
Cyamopsis tetragonoloba (cluster bean), a leguminous plant widely developed in India, has been customarily utilized for different restorative purposes. Preparatory inquire about demonstrates that its cases are wealthy in bioactive compounds with antioxidant and cytoprotective impacts. In any case, its potential part in neurodegenerative maladies such as Parkinson's remains underexplored.
Methods 
•    Plant Extraction: Units were shade-dried, powdered, and extricated utilizing Soxhlet device with ethanol. The extricate was concentrated and put away at 5°C.
•    Phytochemical Screening: Subjective tests distinguished steroids, flavonoids, saponins, terpenoids, and tannins.
•    GC-MS Investigation: GC-MS uncovered compounds such as benzoic corrosive subordinates, siloxanes, and phenolic structures, demonstrating antioxidant potential.
•    In-vitro Cytotoxicity (MTT Test): Neuro-2a cells were treated with shifting concentrations of extricate. Cell reasonability was surveyed by MTT, measuring mitochondrial dehydrogenase action. 
Results: Phytochemical Profile: Nearness of flavonoids, saponins, and phenolic compounds connected to antioxidant and neuroprotective properties.
GC-MS: Recognized 15+ compounds, counting trimethylsilyl subsidiaries and benzoic corrosive esters with potential CNS movement.
MTT Measure: The extricate appeared critical dose-dependent increment in cell reasonability compared to neurotoxin-treated control. IC50 was inside non-toxic run, demonstrating neuroprotection. 
Conclusion: Ethanolic extricate of Cyamopsis tetragonoloba units applies noteworthy in-vitro neuroprotective impacts against initiated neurotoxicity in Neuro-2a cells. The nearness of bioactive phytoconstituents bolsters its restorative potential for overseeing Parkinson's malady. Assist in-vivo and unthinking ponders are justified.