The efficiency of the use of polyphenolic grape processing products in the treatment of experimental metabolic syndrome
Bezrukov S G, Bezrukov G S, Tarimov K O, Ivashchenko N A, Yelcheva L A
For 12 weeks, grape polyphenols were used in 30 Wistar rats on the background of a fructose model of metabolic syndrome. All the animals were on a standard food diet. 3 groups of observations were formed: the 1st "control" (10 animals received 100 ml of 2.5% fructose solution daily); the 2nd "experimental" (10 rats were given the drug "Fanokor" with a total content of polyphenols of 181.53 g/dm3, at the rate of 10-15 ml per 1 animal per day in addition to fructose solution); the 3rd "experimental" (10 animals together with a solution of fructose were given the drug "Enoant" with a total content of polyphenols of 4.20 g/dm3, at the rate of 10-15 ml per 1 animal per day). Animals were measured weekly abdominal circumference and body weight. At the end of the experiment, the morphological structure of the liver, kidneys and abdominal fat was studied in all representatives of the comparison groups. The results were analyzed using the methods of variational statistics and Microsoft Excel software. The statistical analysis was carried out using the Mann-Whitney U-test; the data were considered reliable at p ≤0.05. Microscopic examination of abdominal fat revealed mild and moderate residual diffuse lymphoplasmocytic infiltration in addition to edema, fullness and single petechial hemorrhages. Focal dystrophic changes of hepatocytes were determined, in combination with weakly expressed, mainly periportal, less often diffuse intralobular lymphohistiocytic infiltration and with signs of hemodynamic disorders. The signs of blood circulatory disorders, primarily the fullness of the capillary loops of the glomeruli and single weakly expressed lymphoid clusters in the interstitial, mainly perivascular and in the paranephral tissue stand out during microscopy of the kidney tissues of rats who underwent correction of the metabolic syndrome by "Enoant". The analysis of the obtained results allows us to conclude that the use of grape polyphenols in the composition of "Fanocor" inhibits the development of negative structural and morphofunctional changes in the tissues of the heart, liver, kidneys and visceral cellular in the conditions of modeling the metabolic syndrome, and also optimizes the regeneration in these organs. The obtained data give grounds to recommend polyphenolic products of grape processing for inclusion in the complex of treatment of maxillofacial patients with infectious odontogenic inflammatory diseases developed on the base of metabolic syndrome.
Bezrukov S G, Bezrukov G S, Tarimov K O, Ivashchenko N A, Yelcheva L A. The efficiency of the use of polyphenolic grape processing products in the treatment of experimental metabolic syndrome. International Journal of Pharmacology and Clinical Research, Volume 3, Issue 1, 2021, Pages 1-4