Comparative study of effect of ranitidine and sitagliptin on healing of acetic acid induced gastric ulcer in rats
Amina Unis, Eman Abdelzaher
Gastric ulcer healing is a complex process that is regulated by several promoting factors including Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS). Diabetes mellitus is usually associated with delayed gastric ulcer healing. Hence, the current study was designed to compare the effect of sitagliptin (dipeptidyl peptidase-4 inhibitor) with ranitidine on gastric ulcer healing. The present study was conducted on 40 male albino rats that were divided into four equal groups: Group 1: normal control group, group 2: gastric ulcer model, Group 3: sitagliptin treated group, Group 4: ranitidine treated group. Rats were sacrificed ten days after ulcer induction and stomach was removed for histopathological examination and Immuno histochemical evaluation of COX-2 and iNOS. This study revealed that gastric ulcer healing was significantly impaired in the sitagliptin-treated group when compared with ranitidine treated group, evidenced by histopathological examination of stomach showing significantly larger ulcerated area and ulcer base maturation impairment.COX-2 and iNOS expression as well as mean vascular density (MVD) were significantly diminished in the sitagliptin-treated group as compared to ulcer model group as well as ranitidine treated group. A significant positive correlation was found between COX-2 and iNOS implying their synergistic action. A significant positive correlation was found between MVD on one hand and COX-2 and iNOS on the other hand pointing to their proangiogenic effect. This results raise the question of whether sitagliptin is advisable in diabetic patients with pre-existing gastric ulcer. Our preliminary experimental findings need to be substantiated by future human studies.
Amina Unis, Eman Abdelzaher. Comparative study of effect of ranitidine and sitagliptin on healing of acetic acid induced gastric ulcer in rats. International Journal of Pharmacology and Clinical Research, Volume 2, Issue 1, 2020, Pages 01-08